Conclusions
Throughout this project, with the use of online databases and scientific literature, I have learned how GABRB3 works and what the implications of its function can cause. In order to observe how GABRB3 affects humans, we must first start with a model organism that is simpler to work with, yet still reflects the similarities of the human model. To recap the analyses that were done on the GABRB3 gene and protein:
Homology: Using BLAST and Homologene, different species with conserved GABRB3 protein and DNA were identified and compared to the human model. Several organisms had well-conserved domains when compared to the human model. The most well conserved species were mammals, and of these, I chose the mouse to use as a model organism in my specific aims.
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Chemical Genetics: The chemical genetics screen identified several compounds that affect the expression of GABRB3. Some of the most interesting chemicals were those that are present in many over-the-counter medications. Because the GABA signaling process is so important for the function of the brain, it's interesting that so many chemicals can have an effect on its expression.
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Future directions
Through the chemical genetics screen, we learned that the main compounds used in contraceptive pills can affect the expression of GABRB3. It's also well known that drugs taken by pregnant women can have measurable developmental effects on the child. So how could the consumption of contraceptive pills, or other prescription drugs in general, affect humans in a prenatal environment?
My specific aims focused on the potential effects of nicotine on mouse pups. Expanding on that, it would be interesting to study the effect of other drugs on GABRB3 in the prenatal stages of development. Apart from conducting experiments on mouse models, conducting a Genome Wide Association Study on pregnant women who smoke, or consume prescription drugs.
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